That while cytometry and microscopy allowed for the identification 33 In another study, Leroux and co-workers showed Which is of great interest to the separation industry. Transmission electron microscopy (STEM)-determined particle sizesįound that, equipped with optical microscopy, LD can be used to distinguishīetween spores, vegetative cells, and other particles in a suspension, have also demonstrated a good agreement between LD- and scanning While LD produced similar information in a few minutes. They have found that both techniques produced consistent size information,īut at least 50 microscopy images had to be acquired and analyzedīy optical microscopy to achieve statistically meaningful sampling, Study to measure the size of the cell clumps and pellets of Streptomyces coelicolor using LD and optical microscopy. RønnestĪnd co-workers 36 have conducted a comparison Minimum sample preparation and producing statistically relevant sizeĭistributions significantly faster than microscopic analysis. 8 LD complements microscopy in the analysis ofīiological samples, with the advantage of being nondestructive, requiring Populations, such as fungi, 32 spores, 33 bacteria, 34 cellĬlumps or pellets, 35, 36 and phytoplanktons. More complex biological systems which often contain multimodal particle In using LD alongside microscopy and flow cytometry for measuring Materials, in the last few decades, there has been growing interest Is traditionally used to measure inorganic and synthetic particle 16 It is widely applied in the food, 17− 21 agriculture, 22− 25 construction, 26− 28 and pharmaceutical industries. Typically from 0.1 μm to a few millimeters in size. The size and size distribution of powder particles and colloidal systems, Surrounding media (background) and the non-rigid and non-spherical Of single populations the dynamic nature of the systems a complex Which makes the particles weakly scattering the broad size distribution 10, 11 Other challenges 12− 15 include refractive indexes similar to that of the surrounding medium, Of cells spanning a broad size range from the sub-micrometer level Protein agglomerates 9 and different types The analysis of these particles, forĮxample, in terms of their size distribution, presents a range ofĬhallenges, including the presence of multiple populations such as Surveys, 6− 8 there is an increasing need for analytical toolsĬapable of rapidly measuring critical quality attributes of complexīiological particle samples. The development of advanced therapeutics 3− 5 and encompassing ecosystem This study provides confidence in the use of LD for the measurement The relative concentration of the particle populations in the mixed The method was also found to be useful for estimating Method repeatability was found to be robust, withĭeviations below 1%. Particles larger than 1 μm and generally within 25 % for the Measured by LD for the mixed suspension was accurate within 2 % for We found that the modal diameters of the particle size distribution The selection of the algorithms and validate the measured size distributions. Independent methods were applied to guide We discuss the application of differentĪvailable algorithms to the analysis of the data and their impact Mixed populations of polystyrene particles with diameters rangingįrom 60 nm to 40 μm. Of LD for the measurement of the modal diameter of both single and Of multiple particle populations whose modal size may span across Among theĬhallenges that these types of samples present, there is the presence Measurement of complex biological systems have increased. The results from the PDPA measurements agree very well with the proposed ME tomographic reconstruction.(LD) is a well-established tool for the measurement Experimental validations to the reconstructed results are achieved by using phase Doppler particle analyzer (PDPA). Unlike the classical method of the onion peeling technique or other mathematical transformation techniques that yield unrealistic negative scattered light intensity solutions, the maximum entropy constraints ensure positive light intensity. These reconstructed intensities are in turn converted to local drop size distributions. The tomographic reconstruction, based on the maximum entropy (ME) technique, is applied to forward scattered light signal from a laser beam scanning horizontally through the spray on each plane from the center to the edge of spray, resulting in the reconstructed scattered light intensities at particular points in the spray. This work proposes a new deconvolution technique to obtain local drop size distributions from line-of-sight intensity data measured by laser diffraction technique.
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